PAPER CHROMATOGRAPHY OF STEROIDS

Paper Chromatography of Steroids

In Paper I of this series (1) a method was described for the paper chromatographic analysis of certain ketonic steroids as hydrazones formed with Girard’s Reagent T. When that technique was applied to the adrenal cortical hormones, however, certain deficiencies appeared. (a) The RI values of the individual steroid-Reagent T hydrazones were not sufficiently different to permit identification. (b...

Paper chromatography of C19 steroids.

The successful use of paper chromatography for t.he separation of corticoids (2) suggested the general usefulness of this procedure for relat,ed compounds. Two procedures for t.he separation of cardiac glycosides and aglycones have been described (3, 1). Preliminary reports (5, G) and a reference (7) have been made on methods for other steroid hormones. This paper describes an effective system ...

Quantitative paper chromatography of reducing steroids of the adrenal cortex.

T. MANN AND E. LEONE I953 Neuberger, A. & Webster, T. A. (1946). Biochem. J. 40, 576. Newton, E. B., Benedict, S. R. & Dakin, H. D. (1926). Science, 64, 602. Pauly, H. (1904). Hoppe-Seyl. Z. 42, 508. Pirie, N. WV. (1933a). Biochem. J. 27, 202. Pirie, N. W. (1933 b). Biochem. J. 27, 1181. Potter, Van R. & Franke, K. W. (1935). J. Nutr. 9, 1. Roe, J. H. & Kuether, C. A. (1943). J. biol. Chem. 147...

Glassfiber paper chromatography of adrenal and gonadal steroids.

URRENT METHODS of paper chromatography for separation of steroids from biologic fluids utilize filter paper strips and various partition systems described by Zaffaroni et at. (1) and Bush (2). Chromatography of steroids on alumina-impregnated filter paper was also investigated by Bush (2), but not widely adopted because of certain limitations. Glass-fiber paper impregnated with various adsorben...

Study of phenylketonuria by paper chromatography